According to Chargaff’s rules, which DNA base has the same abundance as adenine?

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According to Chargaff’s rules, thymine has the same abundance as adenine in double-stranded DNA.

The relationship follows complementary base pairing: adenine pairs with thymine, while cytosine pairs with guanine. Therefore, in a typical double-stranded DNA sample, the percentage of adenine equals the percentage of thymine, and the percentage of cytosine equals the percentage of guanine.

Erwin Chargaff and colleagues established these base-composition patterns in the late 1940s and early 1950s. Their observations challenged the idea that DNA was a monotonous, repetitive molecule and helped provide important clues for the double-helix model.

These rules apply to double-stranded DNA, not necessarily to single-stranded DNA or RNA. RNA commonly uses uracil instead of thymine, so substituting uracil into this question would describe a different nucleic acid.

Source: Wikipedia · fact-checked Sept. 2026

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