Who was the first protagonist in the Assassin's Creed series to be a woman?

Aveline de Grandpré was the first woman to serve as a protagonist in the Assassin's Creed series.

Aveline is the central character of Assassin's Creed III: Liberation, originally released for the PlayStation Vita in 2012. She is a Louisiana-born Assassin of French and African heritage who operates in and around New Orleans during the eighteenth century.

The game gives Aveline three disguises, each suited to different social situations and gameplay roles: an Assassin outfit, an adaptable servant disguise, and an elegant lady's dress. Her story addresses slavery, colonial power, and the political conflicts of French Louisiana through the series' fictional Assassin-Templar framework.

Evie Frye later became one of the two main protagonists of Assassin's Creed Syndicate, while Kassandra is a playable protagonist option in Assassin's Creed Odyssey. Aya is also an important playable character in Origins, but Liberation introduced Aveline as the series' first female lead.