Florence is the birthplace of Assassin's Creed II protagonist Ezio Auditore.
Ezio is born in Florence in 1459, and the city is the starting point for his story in Assassin's Creed II. The game presents a fictionalized but recognizable Renaissance Florence, including landmarks such as the Duomo, Palazzo della Signoria, and Ponte Vecchio. Ezio's family life and early missions establish the personal conflict that drives his transformation into an Assassin.
After leaving Florence, Ezio travels through other Italian locations, notably Monteriggioni, San Gimignano, Forlì, Venice, and the Vatican. Venice becomes a major later setting, but it is not his birthplace. Rome is the principal setting of Assassin's Creed Brotherhood, while Forlì is one of the regional locations visited during Assassin's Creed II.
The city also connects Ezio's story to historical Renaissance figures, including Leonardo da Vinci and Caterina Sforza. These appearances mix documented history with the series' fictional Assassin and Templar narrative.