Which game was the first Assassin's Creed title to feature a female protagonist?

Assassin's Creed III: Liberation was the first Assassin's Creed title to feature a female protagonist.

Liberation stars Aveline de Grandpré, a French and African Assassin operating in 18th-century Louisiana. The game was released for PlayStation Vita in 2012, alongside the console release period of Assassin's Creed III.

Aveline's story takes place in and around New Orleans, the Louisiana bayou, and Chichen Itza. Her identity, family background, and opposition to slavery are central parts of the narrative.

Liberation is sometimes overlooked because it began as a handheld spin-off rather than a numbered main entry. Later major games gave players female protagonists or selectable protagonists, including Kassandra in Odyssey and Evie Frye in Syndicate, but Aveline was the earlier featured lead.