Which Assassin's Creed game was the first to feature a female protagonist as one of its two main playable characters?

Assassin's Creed Syndicate was the first main series game to feature a female protagonist as one of its two principal playable characters.

Syndicate stars twin Assassins Jacob Frye and Evie Frye, who operate in London during the Industrial Revolution. Both are central playable characters, and players can switch between them during much of the open-world experience, although some missions are assigned specifically to one twin.

Evie is focused, strategic, and particularly interested in finding Pieces of Eden, while Jacob concentrates on building the Rooks and challenging Templar control over London. Their different abilities encourage distinct approaches to stealth, combat, and exploration.

Earlier Assassin's Creed releases included playable female characters in important roles, especially Aveline de Grandpré in Assassin's Creed III: Liberation. Syndicate is distinguished by placing a female protagonist alongside a male protagonist in a mainline dual-lead structure.