Which Assassin's Creed game is set primarily in the city of Rome?

Assassin's Creed: Brotherhood is set primarily in the city of Rome. Released in 2010, it continues Ezio Auditore's story after Assassin's Creed II and centers on his campaign against the Borgia family and the Templar Order.

Rome serves as a large, interconnected setting in which Ezio liberates districts, recruits and trains new Assassins, and rebuilds the local brotherhood. The game also includes sequences outside Rome, but the city is its defining location and most of the campaign takes place there.

Brotherhood introduced a major recruitment system that lets players call trained Assassins for assistance. It also expanded the modern-day storyline involving Desmond Miles. The title is distinct from Revelations, which follows Ezio to Constantinople, and Unity, which is set mainly in Paris.