Rome is the main city where Assassin's Creed Brotherhood takes place.
The game continues the story of Ezio Auditore da Firenze, who travels to Rome to oppose the Borgia family and rebuild the Assassin Brotherhood. Rome serves as the central open-world area, combining ancient monuments, Renaissance buildings, districts, and surrounding countryside.
Brotherhood follows the events of Assassin's Creed II and expands Ezio's role from individual Assassin to leader. Players can recruit and train Assassins, send them on missions, and call them to assist during gameplay. The restoration of Rome's districts is also a major part of progression.
The historical storyline includes Cesare Borgia and his father, Rodrigo Borgia, whose political power forms the game's main threat. The title is distinct from Revelations, which later moves Ezio's story primarily to Constantinople.