In Assassin's Creed II, Ezio Auditore's birthplace in Tuscany is Florence.
Florence is the opening major setting of Ezio's story and one of the most important locations in the game. Ezio is born into the Auditore family there in 1459, and the city forms the setting for his early life, family tragedy, and first steps toward becoming an Assassin.
The game presents a stylized version of Renaissance Florence, including landmarks such as the Palazzo della Signoria, Santa Maria del Fiore, and the Ponte Vecchio. These places are integrated into exploration, missions, and climbing routes rather than serving only as scenery.
A common mix-up is Venice, which is also a major city in Assassin's Creed II and receives extensive attention later in the story. Florence is Ezio's home city; Venice is a later base of operations. Both are real Italian cities, but only Florence is his birthplace.