Assassin’s Creed Brotherhood is primarily set in Rome, where Ezio Auditore builds an Assassin Brotherhood to oppose the Borgia family.
The game takes place after Assassin’s Creed II and continues Ezio’s story in 1500s Italy. Rome is presented as a large open-world city containing famous landmarks, districts, tunnels, and the Vatican area. Ezio’s main objective is to weaken Cesare Borgia’s control over the city and restore the local Assassin network.
Rome’s role is sometimes confused with Florence or Venice, which are major locations in Assassin’s Creed II. Brotherhood does include brief visits to other places, but Rome is the central playable setting and the location most closely associated with its campaign.
The historical city also supports several game systems, including district liberation, rebuilding shops, recruiting Assassins, and restoring landmarks. These activities connect Ezio’s personal conflict with the broader struggle against Borgia power.