In Assassin's Creed: Brotherhood, Rome is the Italian city controlled by Cesare Borgia and targeted for liberation by Ezio Auditore.
The game takes place primarily in Rome during the early sixteenth century. Cesare Borgia and his allies dominate the city through military power, political influence, and control of its districts. Ezio works with the revived Roman Assassin Brotherhood to weaken that power by eliminating key enemies, destroying military assets, and recruiting allies.
Rome is more than a backdrop: its districts are progressively restored as Ezio removes Borgia control. This structure gives the game a clear liberation theme and distinguishes it from Assassin's Creed II, whose major settings include Florence and Venice. The Vatican and the Colosseum are among the recognizable Roman locations featured in the campaign.
Cesare Borgia was a real Renaissance military leader and nobleman, while the game's secret Assassin-Templar conflict is fictionalized. Brotherhood also introduces a larger role for recruited Assassins and expands Ezio's activities beyond individual missions.