In Assassin's Creed Brotherhood, which historic building serves as the main Assassin headquarters in Rome?

In Assassin's Creed Brotherhood, the main Assassin headquarters in Rome is the hideout on Tiber Island.

Ezio establishes the Roman Brotherhood's base on Tiber Island, a real island in the Tiber River. The location functions as a safe house and management center, where players can access recruits, training, equipment, and several supporting activities.

The headquarters is associated with the Isola Tiberina district and is reached through the game's open-world version of Rome. It is not the Colosseum, Castel Sant'Angelo, or the Pantheon, although all three are important locations that players can visit during the campaign.

Brotherhood expands the series' social and strategic systems by allowing Ezio to recruit and develop Assassin apprentices. Their missions weaken Borgia control across Rome, while improvements to the city generate income and unlock services. Tiber Island consequently represents both a narrative refuge and a practical game hub.