Rome is the city chiefly controlled by the Templars in Assassin's Creed: Brotherhood.
The game continues Ezio Auditore's story after Assassin's Creed II and places most of its action in Renaissance Rome. Cesare Borgia and his allies dominate the city through military, political, and economic power. Ezio works to weaken that control by rebuilding the local Assassin Brotherhood and liberating districts.
Rome functions as the game's main open-world environment. Players can destroy Borgia towers, recruit and train Assassins, restore shops, and reclaim sections of the city. These systems turn the struggle against Templar influence into a visible change in the urban landscape.
Florence and Venice are important locations in Assassin's Creed II, not the primary setting of Brotherhood. Naples appears in the broader historical world of the series, but it is not the principal city that Ezio liberates in this game. The title's focus on a brotherhood refers to the organization Ezio develops in Rome.