In Assassin's Creed: Brotherhood, Rome is almost entirely the game's open-world setting.
Brotherhood continues Ezio Auditore's story after Assassin's Creed II and concentrates its main campaign in Renaissance Rome. Ezio works to weaken the Borgia family's power, rebuild the Assassin Brotherhood, and restore districts controlled by the enemy. The city functions as both a historical setting and a large strategic playground.
Rome contains major landmarks, including the Colosseum, the Pantheon, Castel Sant'Angelo, and the Vatican area. Some locations are represented in compressed or modified form to support exploration, missions, and climbing. The game also introduces recruits who can be trained and sent on missions across Europe.
Brotherhood was the first main Assassin's Creed game to include a dedicated competitive multiplayer mode at launch. Its story remains closely tied to the events of Assassin's Creed II, but its single-city structure gives Rome a more unified role than the multiple-city format used by its predecessor.