In Assassin's Creed: Brotherhood, Ezio Auditore defends and rebuilds Rome.
The game continues Ezio's story after Assassin's Creed II and places him in early-sixteenth-century Italy. Rome is controlled by the Borgia family, whose political and military power has weakened the local population and damaged the city's districts. Ezio works with allies and fellow Assassins to undermine Borgia authority, recruit new members, and restore parts of Rome.
The setting is sometimes confused with Florence or Venice because both cities are prominent in Assassin's Creed II. Brotherhood is more geographically focused: Rome is its principal open-world city, while the surrounding countryside and other areas support the campaign. The title also emphasizes the growth of the Assassin Brotherhood, rather than Ezio working only as an individual operative.