Which character says 'Out, damned spot!' in Macbeth?

Lady Macbeth says “Out, damned spot!” in Shakespeare’s Macbeth.

The line occurs in Act 5, Scene 1, during Lady Macbeth’s sleepwalking episode. She imagines that blood remains on her hands after the murder of King Duncan and the violent crimes that follow. Earlier, she had confidently insisted that “a little water clears us of this deed”; the sleepwalking scene shows that guilt has defeated that apparent certainty.

The “spot” is both a literal imagined stain and a symbol of ineradicable guilt. A doctor and gentlewoman observe her as she relives fragments of the murders, speaks in broken phrases and tries to wash her hands. The scene reverses her earlier role as Macbeth’s forceful instigator: by this point, Macbeth has become the more independent and brutal tyrant, while she is psychologically collapsing.

The quotation is often confused with Macbeth’s “Out, out, brief candle!” speech, but that line appears later in Act 5. Hecate is associated with witchcraft, Lady Macduff is another victim of Macbeth’s tyranny, and Rosalind belongs to As You Like It.