Daisy Duck was first introduced in 1940 in the cartoon Mr. Duck Steps Out.
The short was released on June 7, 1940, and features Donald Duck preparing for a date with Daisy. Her design already included several traits associated with the character, including a feminine voice, a bow, and a strong personality capable of matching Donald’s impatience and energy.
Daisy had earlier development history under related names and designs, which can make references to her first appearance confusing. Mr. Duck Steps Out is generally identified as her first appearance as Daisy Duck, the established character who became Donald’s romantic partner.
Daisy later became a regular member of Disney’s ensemble cast. She is commonly shown as Minnie Mouse’s close friend and as a confident counterpart to Donald, although their relationship is often portrayed through comic arguments, misunderstandings, and reconciliations.